Development of a duplex recombinase-aided amplification assay for direct detection of Mycoplasma pneumoniae and Chlamydia trachomatis in clinical samples

Ming-Zhu Nie,Rui-Qing Zhang,Meng-Chuan Zhao,He Tan,Ya-Xin Hu,Guo-Hao Fan,Jing-Yi Li,An-Na He,Feng-Yu Tian,Feng-Yu Li,Ye-Huan Zheng,Xin-Xin Shen,Yan-Qing Tie,Xue-Jun Ma

Journal of Microbiological Methods（2022）

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摘要

•We developed the duplex-RAA assay to detect Mycoplasma pneumoniae and Chlamydia trachomatis.•We omitted the DNA extraction step.•The Human RNaseP gene as an internal reference reduced the false negatives.•This assay can be performed in a single closed tube at 39 °C within 15 min.•The sensitivity of the duplex-RAA assay was 10 copies/μL.

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关键词

Pneumonia,Mycoplasma pneumoniae,Chlamydia trachomatis,Recombinase-aided amplification,Detection,Internal control

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