Tracking hematopoietic stem cell evolution in a wiskott-aldrich clinical trial
bioRxiv (Cold Spring Harbor Laboratory)(2023)
摘要
Hematopoietic stem cells (HSC) are the cells that give rise to all other blood cells and, as such, they are crucial in the healthy development of individuals. Wiskott-Aldrich Syndrome (WAS) is a severe disorder affecting the regulation of hematopoietic cells and is caused by mutations in the WASP gene. We consider data from a revolutionary gene therapy clinical trial, where HSC harvested from three WAS patients' bone marrow have been edited and corrected using viral vectors. Upon reinfusion into the patient, the HSC multiply and differentiate into other cell types. The aim is to unravel the cell multiplication and cell differentiation process, which has until now remained elusive. This paper models the replenishment of blood lineages resulting from corrected HSC via a multivariate, density-dependent Markov process and develops an inferential procedure to estimate the dynamic parameters, given a set of temporally sparsely observed trajectories. Starting from the master equation, we derive a system of nonlinear differential equations for the evolution of the first-and second-order moments over time. We use these moment equations in a generalized method-of-moments framework to perform inference. The performance of our proposal has been evaluated by considering different sampling scenarios and measurement errors of various strengths using a simulation study. We also compared it to another state-of-the-art approach and found that our method is statistically more efficient. By applying our method to the WAS gene therapy data, we found strong evidence for a myeloid-based developmental pathway of hematopoietic cells where fates of lymphoid and myeloid cells remain coupled, even after the loss of erythroid potential. All code used in this manuscript can be found in the online Supplementary Material, and the latest version of the code is available at https://github.com/dp3ll1n/SLCDP_v1.0.
更多查看译文
关键词
Gene therapy,clonal tracking,Wiskott-Aldrich Syndrome,multivariate Markov process,master equation,generalized method-of-moments,nonlinear differential equations
AI 理解论文
溯源树
样例
![](https://originalfileserver.aminer.cn/sys/aminer/pubs/mrt_preview.jpeg)
生成溯源树,研究论文发展脉络
Chat Paper
正在生成论文摘要