Using Shape Fluctuations to Probe the Mechanics of Stress Granules

Surface tension plays a significant role in many functions of biomolecular condensates, from governing the dynamics of droplet coalescence to determining how condensates interact with and deform lipid membranes and biological filaments. To date, however, there is a lack of accurate methods to measure the surface tension of condensates in living cells. Here, we present a high-throughput flicker spectroscopy technique that is able to analyse the thermal fluctuations of the surfaces of tens of thousands of condensates to extract the distribution of surface tensions. Demonstrating this approach on stress granules, we show for the first time that the measured fluctuation spectra cannot be explained by surface tension alone. It is necessary to include an additional energy contribution, which we attribute to an elastic bending rigidity and suggests the presence of structure at the granule-cytoplasm interface. Our data also show that stress granules do not have a spherical base-shape, but fluctuate around a more irregular geometry. Taken together, these results demonstrate quantitatively that the mechanics of stress granules clearly deviate from those expected for simple liquid droplets. ### Competing Interest Statement The authors have declared no competing interest.