Autophagy Regulation Influences beta-Amyloid Toxicity in Transgenic Caenorhabditis elegans

FRONTIERS IN AGING NEUROSCIENCE(2022)

引用 1|浏览6
暂无评分
摘要
Alzheimer's disease (AD) is a progressive, neurodegenerative disease characterized by the accumulation of amyloid-beta (A beta) proteins in the form of plaques that cause a proteostasis imbalance in the brain. Several studies have identified autophagy deficits in both AD patients and AD animal models. Here, we used transgenic Caenorhabditis elegans to study the relationship between autophagy flux and A beta. We labeled autophagosomes with an advanced fluorescence reporter system, and used this to observe that human A beta expression caused autophagosome accumulation in C. elegans muscle. The autophagy-related drugs chloroquine and 3-MA were employed to investigate the relationship between changes in autophagic flux and the toxicity of A beta expression. We found that reducing autophagosome accumulation delayed A beta-induced paralysis in the CL4176 strain of C. elegans, and alleviated A beta-induced toxicity, thus having a neuroprotective effect. Finally, we used RNA-sequencing and proteomics to identify genes whose expression was affected by A beta aggregation in C. elegans. We identified a series of enriched autophagy-related signal pathways, suggesting that autophagosome accumulation impairs A beta protein homeostasis in nematodes. Thus, maintaining normal autophagy levels appears to be important in repairing the protein homeostasis imbalance caused by A beta expression.
更多
查看译文
关键词
Caenorhabditis elegans, autophagy, RNA-sequencing, quantitative proteomics, Alzheimer's disease
AI 理解论文
溯源树
样例
生成溯源树,研究论文发展脉络
Chat Paper
正在生成论文摘要