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HPLC-PDA法同时测定补肾强筋丸中5种成分

Chinese Traditional Patent Medicine(2022)

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Abstract
目的 建立HPLC-PDA法同时测定补肾强筋丸(山茱萸、续断、白芍等)中莫诺苷、马钱苷、芍药苷、阿魏酸、川续断皂苷Ⅵ的含量.方法 该药物甲醇提取液的分析采用Waters Sun Fire C18色谱柱(4.6 mm×250 mm,5μm);流动相乙腈-0.1%磷酸,梯度洗脱;体积流量1.0 mL/min;柱温28℃;检测波长212 nm.结果 5种成分在各自范围内线性关系良好(r≥0.9997),平均加样回收率97.61% ~99.92%,RSD 1.21% ~1.59%.结论 该方法简便可靠,重复性好,可用于补肾强筋丸的质量控制.
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