Arbuscular Mycorrhizal Fungi Enhanced Drought Resistance of Populus cathayana by Regulating the 14-3-3 Family Protein Genes

The 14-3-3 protein may regulate many biochemical and physiological processes under abiotic stress. Studies have shown that the 14-3-3 protein gene of AMF is not only upregulated under drought stress, but also enhances the regulation of AMF on plant drought tolerance by regulating plant signal pathways and drought response genes; however, knowledge about the biological relevance of these interactions remains limited and controversial. Plants can improve their resistance to a variety of stresses by forming mutualistic relationships with arbuscular mycorrhizal fungi (AMF). The 14-3-3 protein is a major regulator of the plant stress response. However, the regulation mechanism of 14-3-3 family protein genes (14-3-3s) of mycorrhizal plants coping with stress during AMF symbiosis remains unclear. Here, we analyzed the physiological changes and 14-3-3 expression profiles of Populus cathayana inoculated with AMF under different water conditions. The results showed that good colonization and symbiotic relationships with plants were formed under all water conditions (63.00% to 83.67%). Photosynthesis, peroxidase (POD) activity, and Mg and Ca content were significantly affected by drought and AMF. In addition, thirteen 14-3-3 protein genes (PcGRF1-PcGRF13) were identified by quantitative real-time PCR (qRT-PCR), of which the expression levels of PcGRF10 and PcGRF11 induced by AMF were significantly positively correlated with superoxide dismutase (SOD), POD, and sugar content, indicating that the 14-3-3s of mycorrhizal symbiotic plants may respond to drought through antioxidant and osmotic regulation. This is the first study on 14-3-3s in the symbiosis system of forest arbor plants and AMF, and it may help to further study the effects of 14-3-3s during AMF symbiosis on stresses and provide new ideas for improving mycorrhizal seedling cultivation under stress. IMPORTANCE The 14-3-3 protein may regulate many biochemical and physiological processes under abiotic stress. Studies have shown that the 14-3-3 protein gene of AMF is not only upregulated under drought stress, but also enhances the regulation of AMF on plant drought tolerance by regulating plant signal pathways and drought response genes; however, knowledge about the biological relevance of these interactions remains limited and controversial. The precise functions of Populus cathayana 14-3-3s under drought stress remain poorly resolved and the mechanisms of action of these genes in mycorrhizae-induced drought stress are still unknown. Thus, studying the drought-resistance mechanism of the AMF symbiotic plant 14-3-3 gene is of special significance to improving the drought tolerance of the plant. Further systematic study is needed to probe the mechanism by which AMF regulates different 14-3-3 genes and their subsequent physiological effects on drought.