Feasibility study of biohydrogen production using Clostridium sartagoforme NASGE 01 from cassava industry effluent

The present study demonstrated the production of biohydrogen from the cassava industry wastewater and the formation of organic acids. The biohydrogen production was carried out with Clostridium sartagoforme NASGE 01, an anaerobic bacterium which could directly utilize the carbon source under anaerobic condition. The best result of hydrogen production was obtained at optimum fermentation condition of pH 5 and temperature 35 °C. For the enhancement of the biohydrogen production, the fermentation media enriched with carbon and nitrogen sources. The maximum hydrogen yield obtained from starch (6 g/L) was 0.8 mol H 2 /mol glucose and starch (6 g/L) with yeast extract (3 g/L) was 0.91 mol/mol glucose with the substrate degradation efficiency of 42.6 and 40%, respectively. Moreover, the modified Gompertz curve fit model equation was applied for the kinetic study of experimental data and found out the hydrogen production potential and hydrogen production rate ( Rm ). In addition, liquid phase of reactors was detected by gas chromatography equipped with flame ionization detector and the major soluble metabolites generated during fermentation were found to be butyric and propionic acid. The experimental data revealed that producing hydrogen from cassava wastewater by using pure culture of C. sartagoforme NASGE 01, thereby decreasing the deleterious properties of effluent from starch-based industries and developing the environmental sustainability. Graphical abstract