Effect of Pediatric Toothpastes Based on 500 to 1450 ppm Sodium Fluoride and Amine Fluoride with Different Detergents on oxidative Stress and Cell Viability

Objective: This study evaluated the effect of sodium fluoride and amine fluoride pediatric toothpastes with different detergents on oxidative stress and cell viability. Materials and Methods: Pediatric toothpastes containing sodium fluoride (Sensodyne Pronamel-SP; Ipana Kids-IK; Signal Kids-SK; Oral B-OB) and amine fluoride (Elmex-EL; Elmex Junior-EJ) were obtained. While SP, IK, EL contained cocamidopropyl betaine detergent, SK, OB sodium lauryl sulfate and EJ had olaflur detergents. Toothpaste samples were diluted with medium at different predetermined concentrations. L929 fibroblast cells were exposed to toothpaste extracts at 37 degrees C for 2 min. Cell viability was tested using methyl tetrazolium test, while the formation of reactive oxygen species (ROS) was detected using flow cytometry. Results: The decreasing concentration ratio from 1:1 to 1:32 decreased the cytotoxicity (p<0.05) except EL. The cytotoxicity of original pediatric toothpaste extracts (1:1) were significantly different, compared to the negative control group (p < 0.05), except IK and EL (p > 0.05). All toothpastes tested increased the number of ROS in L929 cells (p<0.05). Conclusion: Pediatric toothpastes containing sodium fluoride with sodium lauryl sulfate presented more cytotoxic effect.