High fruit setting rate without male participation: A case study of obligate apomixis in Rhomboda tokioi (Orchidaceae)

•The data of breeding experiments, flora morphology, pollinators visitation indicated R. tokioi produces seeds through obligate diplosporic apomixis, which is the first case of obligate diplosporic apomixis in orchids.•The observation of embryo sac and embryo dynamic development revealed apomixis in this orchid involves the unreduced embryo sac and parthenogenesis of egg cells, and its apomixis model might be Allium - type diplospory.