Dipeptidyl peptidase-IV inhibitory potential of alpha-lactalbumin extracted from milk of Gir cows: A Bos indicus species

An attempt has been made to assess the dipeptidyl peptidase-IV (DPP-IV) inhibitory potential of alpha-lactalbumin separated from milk of Gir cow. alpha-lactalbumin hydrolysates were prepared using three different enzymes at different enzyme-to-substrate (E:S) ratios (1:25, 1:50 and 1:100) treated for 2- to 12-h durations. Pepsin-treated hydrolysates showed maximum DPP-IV inhibition of 87.81 +/- 0.84% (IC50 = 0.78 mg/mL). The permeate of 3 kDa, which exhibited 84.47 +/- 2.29% DPP-IV inhibition, was fractionated through RP-HPLC. All fractions were analysed for DPP-IV inhibition activity. One novel peptide was identified through LC-MS/MS, which showed adequate DPP-IV inhibition (IC50 = 4.93 mM).