Expanding signaling repertoire of anoctamin 1 by localization at membrane contact sites

IRBIT (Inositol 1,4,5-Trisphosphate (IP3) Receptor Released with IP3) is an inhibitor of the IP3 receptors and Ca2+ signaling and IRBIT regulates and activates several transporters and ion channels, to coordinate neuronal function and secretory glands fluid secretion. Our present work asked whether and how IRBIT affects the function of (TMEM16A, ANO1) in vivo and in vitro. Our findings indicate that IRBIT mediated epithelial transport in vivo via expanding signaling repertoire, revealing a synergy mechanism between cAMP and Ca2+ signaling.