TRamWAy: mapping physical properties of moving biomolecules in large scale single-particle tracking experiments

Single-molecule localization microscopy allows studying the dynamics of biomolecules in cells and resolving the biophysical properties of the molecules and their environment underlying cellular function. With the continuously growing amount of data produced by individual experiments, the computational cost of quantifying these properties is increasingly becoming the bottleneck of single molecule analysis. Mining these data require an integrated and efficient analysis toolbox.