Intracellular metmyoglobin detection using fluorescence lifetime imaging of various YFP-myoglobin-mCherry constructs

Myoglobin is expressed unevenly among cell varieties, and it has only recently been found in some types of cancer cells. Nevertheless, it has been widely acknowledged that the Fe3+ state of myoglobin, metmyoglobin (metMb) has a broad functional role in metabolic pathways, oxidative/nitrative regulation and gene networks of many cells. Accordingly, real-time monitoring of oxygenated, deoxygenated and metMb proportions- or, more broadly, the mechanisms by which metMb is formed, is a promising field of research.