Long time observation of a single lipid on phase separated membrane

In live cell, plasma membrane consisted of separated phases which is in dynamic equilibrium. The phase separation regulate behaviors of the biomolecules as well as downstream signaling. Due to the sub-diffraction limited size (< 200 nm) of the viscous nanodomain (i.e., lipid raft), the molecular time trace at a high space-time accuracy is important for visualization of multiphase nature of the membrane. We analyze individual molecular motion with high localization accuracy by a single molecule localization microscopy (SMLM) and the hidden Markov’s model (HMM).