Effect of glycerol pathway on cholesterol degradation in a Mycobacterium model strain

The glycerol metabolic pathway plays an important in the growth and infection of the pathogenic Mycobacterium H37Rv strain, which has not been explored. In this work, we utilized a non-pathogenic Mycobacterium HGMS2 as a model strain to investigate how the glycerol metabolic pathway affects its growth. Based on our recently-determined whole genome sequencing data on the HGMS2 strain, we predicted the glycerol metabolic pathway and two key enzymes in this non-pathogenic model strain. The two key enzymes, namely, alcohol dehydrogenase [adhc, EC: 1.1.1.2] and glycerol kinase [glpk, EC: 2.7.1.30] divided the HMGS2 glycerol metabolic pathway into two branches.