Multiplexed two-color imaging and analysis reveals the influence of actin cortex dynamics on CD36 receptor organization

CD36 is a cell surface receptor whose organization and signaling in multiple cell types have been shown to depend on the actin cortex, although largely through actin perturbation experiments. Perturbation experiments raise concerns of nonspecific effects, and preclude any quantification of actin dynamics. Thus, to derive explicit relationships between cell surface CD36 organization and actin cortex dynamics simultaneously in live unperturbed cells, we developed a novel imaging and computational analysis framework capable of integrating single-molecule imaging of receptor behavior and fluorescent speckle microscopy of actin cortex dynamics (SMI-FSM).