Automated 3D reconstruction of isolated mitochondria from cardiomyocyte SBEM images

Mitochondrial morphological defects are often reported in pathologies associated with cardiac dysfunction, such as cardiomyopathies, diabetes, neurodegeneration, aging, and cancer. However, no causative role has been established between the defects and pathologies. Quantitative assessment of mitochondrial morphology is important for investigating the mechanisms but is limited because of laborious manual segmentation of electron micrograph (EM) images. The existing automated method does segment mitochondria from myofibril but doesn't separate each mitochondrion which is necessary for evaluating fragmentation or dilation.