Coralline Hydroxyapatite Coated with a Layer Biomimetic Calcium Phosphate Containing BMP-2 Induces Dose-Related Ectopic Bone Formation in Wistar Rats

In order to evaluate loading methods and the dose dependency of bone morphogenetic protein 2 (BMP-2) in ectopic bone formation, an osteoinductive material consisting of commercially available coralline hydroxyapatite (CHA) was coated with a layer of biomimetic calcium phosphate (BioCaP) containing BMP-2 in different ways. Eight groups-each containing samples of 0.25 g CHA-were formed and coated with, respectively, BioCaP with internally incorporated BMP-2 in concentrations of 1, 5, 10, 20, 40 and 60 mu g per sample, and the two control groups with BioCaP only and BioCaP with 20 mu g of adsorbed BMP-2 per sample. The samples were implanted subcutaneously in 27 male Wistar rats. The histological results show that there is no bone formation in the group in which no BMP-2 was included. All samples with BioCaP containing BMP-2 show bone formation. The group with 20 mu g of adsorbed BMP-2 per sample shows the least bone formation. Coating-incorporated BMP-2 is more efficient in inducing bone formation than adsorbed BMP-2. The group with 5 mu g of coating-incorporated BMP-2 per sample shows the most bone formation. Increasing the amount of coating-incorporated BMP-2 up to 60 mu g does not improve ectopic bone formation.