Studies on Anemone nemorosa L. extracts; polyphenols profile, antioxidant activity, and effects on Caco-2 cells by in vitro and in silico studies

In this study, two polar extracts (aqueous and ethanolic) from the aerial part of Anemone nemorosa L. (dried plant) were assessed to reveal their polyphenols profile, antioxidant activity, cytotoxic, and antiproliferative activity on Caco-2 (ATCC-HTB-37) cell line. In silico studies on two key compounds, protoanemonin and anemonin, on four molecular targets - cyclooxygenase-1 and cyclooxygenase-2, and human tankyrase 1 and human tankyrase 2 in relation to human colon cancer cell development have also been achieved. The results are as follows: caffeic acid esters and quercetin glycosides, including (iso)rhamnetin derivates, are the major polyphenol compounds in wood anemone polar extracts; the two polar extracts indicated very strong antioxidant activity in the interval from 0.1 to 5 mu g [GAE] per 1 mL sample (IC50 < 0.290 mu g GAE/mL), and in vitro studies on Caco-2 cells have revealed their simultaneous stimulatory and protective activity exactly in the concentration area with the strongest antioxidant activity. In silico studies have revealed moderate inhibitory activity of the two key compounds, anemonin and protoanemonin, on the four molecular targets studied; it was concluded having particular benefits of the wood anemone polar extracts in managing postoperative intestinal recovery, and generally in regenerative medicine.