CmMYB1 gene clone from Cucumis melo and its functional roles under salt stress

This study aims to clone the MYB gene from the melon and explore its function. In order to obtain the differentially expressed MYB genes, comparative transcriptome analysis was carried out between varieties 'M15' and 'Baogua'. CmMYB1 homologous gene in muskmelon (Cucumis melo L.) was obtained by homologous cloning. CmMYB1 gene was introduced into wild type by agrobacterium-mediated transformation, and the growth status of T2 transgenic and wild type was observed. The results of subcellular localization showed that the CmMYB1 protein was expressed in cytoplasm and nucleus. After treatment with 200 mM NaCl solution for 1 and 3 h, the CmMYB1 gene expression increased significantly and began to decline after 6 h of salt stress, indicating that it functions in the early response to salt stress. After treatment with 200 mM NaCl solution for 1 and 3 h, the CmMYB1 gene expression increased significantly, and began to decline after 6 h of stress, indicating that it functions in the early response to salt stress. In CmMYB1-OX overexpression Arabidopsis and Arabidopsis Col-0 treated with 200 mM NaCl solution for 14 days, the overexpression lines at L4 and L5 were seen to change more obviously, and these results suggested that the CmMYB1 gene was involved in the response to salt stress.