Effects of Lactobacillus Rhamnosus on dental implant osseointegration in osteoporotic rabbit model

Aim: The effect of Lactobacillus rhamnosus (LR), probiotic bacteria that manage inflammatory disease, on osseointegration is unknown. The study aimed to evaluate the effects of LR administration on osseointegration in experimental osteoporosis induced by ovariectomy. Material and Methods: Sixteen New Zeland female rabbits were randomly divided into the following groups: SHAM (SH), SHAM and LR (SH+LR), Ovariectomy (OVX), OVX and LR (OVX+LR). Animals in the OVX group were subjected to bilateral ovariectomy to create artificial osteoporosis. After 16 weeks, a titanium dental implant was bilaterally implanted into each tibia of the animals. Animals in the SH+LR and OVX+LR groups were given oral probiotics. All animals were sacrificed and all tibia bones were separated at postoperative 8 weeks after implantation. Cardiac blood was taken to determine biochemical markers. Resonance frequency analysis (RFA) and bone-to-implant contact area (BIC) were administered. Results: RFA and BIC area were statistically significant for osseointegration in OVX+LR compared with OVX (p<0.05). The BIC area in the OVX group was statistically lower (p<0.05). ALP, BALP, Trap5b and MPO were highest in OVX groups(p<0.05). Discussion: This study concluded that osseointegration may be improved by LR administration in an estrogen-deficient state. The success rate of the implant may be increased by using probiotics in patients with osteoporosis.