Cryoprotectant-free vitrification of llama spermatozoa: cryoloop vs sphere method, warmed rapidly or ultra-rapidly
Small Ruminant Research（2022）
Abstract
•Vitrification of Lama glama sperm, conserves chromatin condensation and integrity.•Best results were obtained using the spheres method without cryoprotectants.•Best warming method: 37 °C for 30 s.
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Key words
Spermatozoa,Vitrification,Cryoloops,Spheres,Lama glama
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