Research on the effect of Deinococcus radiodurans transformed by dsrA-flr-2 double gene on the enrichment performance of uranium(VI)

Strong-reducing gene ( dsrA ) and the fluorine-resistant gene ( flr-2 ) were cloned into D. radiodurans to prepare a recombinant Deino-dsrA-flr-2 strains . Then, the influencing factors of uranium enrichment by Deino-dsrA-flr-2 and its enrichment mechanism were studied. When the initial concentration of uranium was 30 mg/L, the pH was 5, the biomass was 0.016 g and the enrichment time was 75 min, the Deino-dsrA-flr-2 had the best remove effeciency of U(VI). SEM and TEM could clearly observe uranium deposits on the surface of bacteria; EDS also indicated that uranium appeared on the surface of Deino-dsrA-flr-2 . Compared with D. radiodurans , Deino-dsrA-flr-2 can better remove uranium.