Studying the Specific Localization of Toothrin Protein from Related D4 Family in Drosophila melanogaster

In this work, the expression pattern of the TTH protein, which is related to the D4 protein family, was studied at the larval stage of Drosophila melanogaster development for the first time . For this purpose, polyclonal antibodies to the TTH protein and a Drosophila line of flies transformed with a genetic construct containing a modified tth locus for the expression of the GFP-tagged TTH::GFP protein have been obtained. Analysis of the expression patterns of native and labeled proteins showed the localization of TTH in the nervous system, specialized endocrine glands, and salivary glands of larvae, as well as in the structures that form the innervation of the eyes of an adult insect. It has been suggested that the tth gene is involved in the development of the nervous and visual systems, as well as in the functioning of organs responsible for hormonal regulation and digestion.