Evaluation of Excreta/Secreta of Lucilia sericata Larvae as a New Antibacterial Candidate for Treatment of MRSA Ocular Infection
BIOINTERFACE RESEARCH IN APPLIED CHEMISTRY(2022)
摘要
Staphylococcus aureus is the most serious cause of bacterial keratitis in most populations. Due to the resistance of this bacteria to methicillin (MRSA), the novel antimicrobial component is required for novel treatments for keratitis. Excretions/secretions (ES) from larvae of Lucilia sericata (maggot) have antibacterial activity against a number of bacteria. Around 500 larvae were washed with 1 mL of sterile water every hour for ES development. Bactericidal activity of ES was examined by time killing assay. MRSA strain was injected intrastromal into rabbit corneas. The rabbits have been split into three categories, including artificial tear, gentamicin, and maggot ES. Following, the eyes were clinically examined by a slit lamp. This evaluation included the conjunctiva, iris, and cornea. Lastly, the cornea of rabbits was collected for bacterial colony counts. After statistical analysis by ANOVA, it was found that gentamicin and ES, significantly reduced ocular infections in rabbits. Comparisons of corneal opacity scores with Mann-Whitney U-test showed a decreasing trend of therapeutic effects as shown below: gentamicin>ES>control. Gentamicin and ES significantly decrease CFU (number of colony-forming units) compared with the control. The mean bacterial count (log CFU/mL) from corneal culture for ES, gentamicin, and untreated groups were 6.04, 5.0, and 8.9, respectively (P <= 0.05). In conclusion, the extraction of maggot larvae is useful in treating keratitis mediated by MRSA.
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关键词
Staphylococcus aureus, maggot, antibacterial, ocular infection
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