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Coupling nucleic acid circuitry with the CRISPR-Cas12a system for universal and signal-on detection

Rujian Zhao,Chunxu Yu,Baiyang Lu,Bingling Li

RSC ADVANCES（2022）

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Abstract
We report a universal and signal-on HCR based detection platform via innovatively coupling the CRISPR-Cas12a system with HCR. By using this CRISPR-HCR pathway, we can detect different targets by only changing the crRNA. The CRISPR-HCR platform coupling with an upstream amplifier can achieve a practical sensitivity as low as similar to aM of ASFV gene in serum.
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