Phytochemistry and anti-inflammatory activity of iridoids from Dolichandrone spathacea collected in the mangrove forest of Phu Loc district, Thua Thien Hue province, Vietnam

From the leaves and barks of Dolichandrone spathacea collected in the mangrove forest of Hue province, three iridoids {6-O-[(E)-4-methoxy cinnamoyl]catalpol (1), 6-O-[(E)-3,4-dimethoxy cinnamoyl]catalpol (2), nemoroside (3)} and three ursane-type triterpenoids [ursolic acid (4), pomolic acid (5), uncaric acid (6)] were isolated and structurally elucidated. Besides, succrose octaacetate (7), daucosterol tetraacetate (8) and 1-hentriacontanol (9) were identified from the fruits of this mangrove species. It is the first time compounds 6, 7, 9 were isolated from Dolichandrone spathacea. The main constituents of this plant, iridoids 1 and 2, were tested for the inhibition of IL-6, TNF-alpha factors and NO production. The anti-inflammatory activity as well as the phytochemistry of the fruits of this species are reported for the first time.