Spectroscopic features of Pheophorbide-a binding to poly-L-lysine
MOLECULAR CRYSTALS AND LIQUID CRYSTALS(2022)
摘要
Binding of Pheophorbide-a of to poly-L-lysine (PLL) have been studied in neutral aqueous buffered solutions of low and near-physiological ionic strengths with low ethanol content (2.4-5.9%) in a wide range of molar polymer-to-dye ratios (P/D) using absorption and polarized fluorescence spectroscopy, fluorimetric titration, fluorescence melting. The binding has highly cooperative character (cooperativity parameter q approximate to 1000) and results in 40-fold quenching of the dye fluorescence as well as in increase in the fluorescence polarization degree up to 0.16, that evidences formation of pi-stacked dye aggregates on the polypeptide exterior. The spectroscopic properties of these aggregates were established. The cooperative biding constants were estimated by Schwarz's method.
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关键词
absorption, fluorimetric titration, melting, Pheophorbide-a, polarized fluorescence, poly-L-lysine, thermodynamic binding parameters
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