Optimization of physicochemical parameters and characterization laccase enzyme produced by a novel strain of Fomes fomentarius through solid-state fermentation

This present study presents that high yield of laccase (260 +/- 1.8 U/mL) were obtained by indigenous fungal strain of Fomes fomentarius produced after 10th day of incubation at 35 degrees C using corn stover as a substrate. Furthermore, laccase was precipitated by 50% saturation with ammonium sulfate having 1.9 purification-fold after dialysis. Purification factor increased up to 2.2-fold (1.65 U/mg specific activity) with Sephadex G-100 gel filtration chromatography. The molecular weight of laccase was 44 kDa when estimated with sodium dodecyl sulfate polyacrylamide gel electrophoresis. Laccase maximum activity (230 +/- 3 U/mL) was obtained at pH 7 and increased at 35-40 degrees C up to (258 +/- 2.8 U/mL). The kinetic studies of laccase showed low Km (1 mM) with high Vmax values (60 mM/min). Purified laccase at a concentration of 0.25-1.00 mL/mL of lignin led to 80-90% lignin degradation. Laccase produced from Fomes fomentarius shown good decolorization properties (82%). Thus, Fomes fomentarius laccases can potentially be utilized in food, textile, pulp, and paper industries and also used in waste decontamination.