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Lattice-like 3-dimensional DNA nanostructure-based bioseparation strategy for highly accurate quantification of low-abundance cancer biomarker via mass spectrometry

Rui Zhai,Zhanying Chu,Manman Zhu,Lixing Yu,Ximei Lei,Tao Peng,Yang Zhao,Xiaoyun Gong,Jie Xie,You Jiang,Liqing Wu,Weiqi Rong,Xinhua Dai,Xiang Fang

Chemical Engineering Journal（2022）

Cited 6|Views9
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Abstract
•A 3-dimensional DNA tetrahedron carrier was used for precise antibody arrangement.•The composites exhibited ideal performance in the capture of low-abundance HSP90α.•The novel strategy has great potential in quantification of other protein biomarkers.
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Key words
DNA tetrahedron,Magnetic nanoparticles,Immunoaffinity enrichment,Low-abundance proteins,MS quantification
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