The Effect of Diammonium Glycyrrhizinate Lipid Ligand on Nonalcoholic Fatty Liver Disease Induced Leukocyte Infiltration in Rat Model

Objective: To investigate the effects of diammonium glycyrrhizinate lipid ligand (DGLL) on nonalco-holic fatty liver disease (NAFLD)-induced leukocyte infiltration in rat and underlying mechanisms. Methods: Sixty SD rats were randomly distributed into six groups, control group, NAFLD model group, DGLL (30, 60, 120 mg/kg) treatment group and positive control (biphenyl biester tablets (BDP), 200 mg/kg) group. The expression and activity of myeloperoxidase (MPO) in liver tissues were measured by immunohistochemistry and ELISA, respectively. The expressions of leukocyte adhesion molecules in whole blood were determined by flow cytometry. The intercellular adhesion molecule-1 (ICAM-1) expressions in liver tissues was detected by western blotting. Results: Com-pared with NAFLD model groups, the expression and activity of MPO in liver tissues of DGLL (30, 60, 120 mg/kg) treatment group were all significantly ameliorated by the administration of DGLL (P < 0.05). Meanwhile, the up-regulation of adhesion molecules expressed on monocytes and neu-trophils in whole blood, as well as endothelial ICAM-1 expression in NAFLD model groups were eliminated by DGLL treatment (P < 0.05). Conclusion: DGLL ameliorated leukocyte activation and infiltration in rat liver with NAFLD, which might be related to suppression of adhesion molecules IP: 49.249.253.194 On: Fri, 25 Mar 2022 08:19:18 expression on leukocyte and endothelium. Copyright: American Scientific Publishers