Two-photon fluorescence depolarization dynamics of ionic strength biosensors
Biophysical Journal(2022)
摘要
Compartmentalized, dynamic ionic strength within living cells influences numerous biochemical mechanisms such as catalytic function, protein folding, osmotic pressure, and energy production. Recent development of genetically encoded donor-linker-acceptor biosensors that undergo Förster resonance energy transfer (FRET) offers a promising methodology towards noninvasive, site-specific, quantitative, and sensitive mapping of in vivo ionic strength. Here, we investigate the effects of amino acid sequence in the flexible linker on the FRET efficiency and hence the sensitivity to ionic strength of varying KCl concentration.
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关键词
ionic strength biosensors,fluorescence,two-photon
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