Simulations of fluorescence fluctuation spectroscopy measurements of molecular diffusion in biomembranes

Studying the diffusion of individual molecules can yield important insights into the complex organization and function of the cell plasma membrane. Fluorescence fluctuation spectroscopy techniques allow the study of diffusion with single moleculesensitivity. Here, we present a novel, versatile and open accessible program based on Python for simulating molecular membrane diffusion. There have been different approaches to describe the diffusion laws governing the experimentally measured complex trajectories at the plasma membrane of live cells.