Wavefront shaping with Hadamard basis for soil imaging

Soil is a highly scattering media that inhibits imaging of plant-microbial-mineral interactions that are essential to plant health and soil carbon sequestration. However, wavefront shaping can be used to focus light through or even deep inside highly scattering objects. In this work, we seek to overcome the fundamental challenges of imaging through soil minerals by developing a custom wavefront shaping method for a multiphoton microscope. We use the adaptive stochastic parallel gradient descent optimization algorithm combined with Hadamard basis to correct the aberration and the scattering in order to focus through the soil.