Preparation of SARS-CoV-2 VLP to study viral assembly, egress, and entry
Biophysical Journal(2022)
Abstract
Virus-like particles (VLPs), assembled by expressing viral structural proteins in cultured cells, exhibit similar morphology to intact viruses. SARS-CoV-2 (CoV2) VLPs would be a useful tool to safely study viral assembly, release, and entry into target cells without the biohazards of replicating virus. We evaluated three strategies for producing CoV2 VLPs: 1) minimal CoV2 VLPs, by co-expressing the four CoV2 structural proteins: Spike (S), Nucleocapsid (N), Membrane (M), and Envelope (E) in HEK293T cells, 2) influenza VLPs pseudotyped with the CoV2 S protein, and 3) chimeric VLPs expressing a chimeric CoV2 S protein consisting of the S ectodomain fused to the transmembrane and cytoplasmic tail of influenza hemagglutinin.
MoreTranslated text
Key words
viral assembly,sars-cov
AI Read Science
Must-Reading Tree
Example
![](https://originalfileserver.aminer.cn/sys/aminer/pubs/mrt_preview.jpeg)
Generate MRT to find the research sequence of this paper
Chat Paper
Summary is being generated by the instructions you defined