Innovative nanomaterials for bone remains consolidation: performance evaluation and impact on 14C dating and on palaeogenetic analysis

An innovative protocol for the consolidation of ancient bone remains based on the use of nanometric hydroxyapatite (HAP) was set up and tested through a multidisciplinary approach. A new protocol for the synthesis of HAP nanoparticles was developed, and the composition of the obtained nanomaterial were investigated through Fourier Transform Infrared Spectroscopy (FTIR) and X-Ray Diffraction (XRD); sizes, shape and morphology of the synthesized particles were studied by Scanning Electron Microscopy (SEM). The consolidation performance was evaluated by testing the new nanomaterial on degraded ancient bone findings. An increase of the mineral density and of the micro-hardness of the bone were observed. The new consolidation method was also tested to assess possible effects on the palaeogenetic analysis and radiocarbon dating on the treated bones. The consolidation treatment has no substantial impact on the genetic characterisation of the skeletal remains and does not introduce any contaminations that could affect radiocarbon dating. This consolidation procedure represents a more compatible conservation tool with respect to traditional procedures: it has been shown that the treatment is effective, easily-applicable and not detrimental for 14C dating and palaeogenetic analysis.