Addition of Gourami Fish Scales to Glass Ionomer Cement (GIC) Increases Odontoblast like Cells Number and Expression of IL-10 (Interleukin-10)

Introduction. Fish scales are usually not used anymore or thrown away, in fact they contain organic and inorganic materials that are also found in bone and dentin. Purposed. This research to analyze the addition of gouramy scales powder (GSP) to Glass Ionomer Cement (GIC) on odontoblast count and IL-10 expression in the oral cavity of Wistar rats. Method. we used male Wistar rats C: group mice that did not bore holes in their teeth. Groups in this research, P1: the sample is filled with GIC. P2: GIC + 2.5% GSP. P3: GIC + 5% GSP. P4: GIC + 10% GSP. After 7 days the rats were sacrificed and their teeth were made preparations for odontoblast cells observation (light microscope magnification 400 times) and analysis of IL-10 by immunohistochemical technique. Results. The ANOVA and LSD test confirmed significant differences. The increase in the number of odontoblasts and IL-10 is thought to be due to goramy scales caused by chitin, calcium, amino acids, omega 3, and omega 6. Conclusion. The addition of gouramy scales powder (GSP) to Glass Ionomer Cement (GIC) increased the number of odontoblasts like cells and the expression of IL-10 in the oral cavity of Wistar rats. So that the addition of gouramy fish scales can be considered to improve the quality of GIC.