dsDNA quantification using Sybr Green I v2

This is a simple protocol that uses Sybr Green 1 and a microplate reader to quantify dsDNA concentrations in unknown samples. This is best suited for situations where a high number of samples need to be quantified (e.g. normalisation of samples before pooling for Illumina sequencing). For smaller sample numbers (e.g. < 50 samples) it will be more efficient to simply use a qubit or nanodrop. You need to pick an appropriate standard concentration range for the type of samples you will be testing. For genomic DNA it is probably better to have a lower concentration range (0-10 ng/μl) whereas for PCR products, standards will probably need to be much more concentrated (0-50 or maybe even 100ng/μl).