Development of an analytical method to determine E7130 concentration in mouse plasma by micro-sampling using ultra-performance liquid chromatography–high resolution mass spectrometry

•E7130 is a novel microtubule inhibitor and a promising ameliorator of the tumor microenvironment.•An analytical method was developed to quantify E7130 in mouse plasma.•A sensitive method was established at a LLOQ of 0.2 ng/mL for plasma microsamples (10 µL).•HRMS provided a high selectivity for quantification of E7130 in the biological matrix prepared via protein precipitation.