Characterization of the mechanisms of action and mosquito larva midgut response to a yeast- encapsulated orange oil larvicide

Abstract Here, we assessed the mechanism of action of a yeast-encapsulated orange essential oil (YEOO) larvicide. YEOO is readily ingested by mosquito larvae leading to midgut epithelial cell responses associated changes in gene expression profiles and severe epithelial damage that were detected shortly after YEOO ingestion. In Aedes aegypti 3 rd instar larvae exposed to YEOO at LC90, greater than 10 fold induction of the effector caspase CASPS8, >11 fold induction of the midgut epithelial regenerator Vein , and >26 fold induction of the inhibitor of apoptosis gene IAP1 compared to control larvae by 8 h after YEOO ingestion. For sublethal concentrations (更多