High Expression of ISYNA1 Preidicts Unfavourable Prognosis in Patients with Colorectal Cancer

Background: Mining the prognostic biomarkers of colorectal cancer (CRC) has important clinical and scientific significance. Inositol-3-phosphate synthase 1 (ISYNA1) has been reported in very few previous literature and its role in cancer remains controversial. However, the prognostic role of ISYNA1 in CRC still remains to be characterized. Methods: The potential prognostic factor ISYNA1 was screened out through TCGA database analysis. Then its expression and associations between clinicopathological variables were assessed in TCGA CRC cohort. The prognostic value of ISYNA1 was assessed by multiple methods, such as ROC curve, Kaplan-Meier method and univariate/multivariate Cox regression analysis. Then, functional enrichment and annotation among the high and low ISYNA1 groups were achieved by GO and KEGG analysis and GSEA. CRC tissue microarray and fresh CRC tissue samples obtained clinically were used for further validation.Results: ISYNA1 was highly expressed in CRC tissues compared to normal tissues. Meantime, overexpression of ISYNA1 correlated with higher N stage, M stage, AJCC stage, more lymphatic invasion and more R2 resection. Besides, high expression of ISYNA1 was closely correlated to less favorable OS, DSS and PFI. Moreover, ISYNA1 was identified an independent prognostic factor of CRC by performing univariate/multivariate Cox regression analysis. The analysis of CRC tissues microarray further confirmed the conclusion drawn from TCGA data analysis.Conclusion: ISYNA1 is notably upregulated in CRC tissues and may serves as a potential biomarker of CRC.