Jigsaw-like mini-pillar platform for multi-mode biosensing

The multiple sensing provides booming options to eliminate interference and ensure the accuracy of detection by mutually coupling and validating multiple data sets. Here, we integrate the jigsaw-like multifunctional mini-pillar platform to perform multi-mode (electrochemical, fluorescence, surface-enhanced Raman scattering (SERS) and colorimetric) sensing in individual microdroplets. Each mini-pillar connector can parallelize together by specific concave-convex interface to form integrated jigsaw-like platform for multi-mode sensing, and each specific mini-pillar can be modified into the individual sensing unit to read the prescribed signals. We successfully implemented electrochemical, fluorescence, SERS and colonmetric detection by multiple signals coupling to reduce the false positive analysis. Such platform brings a promising clue of in situ analysis and point-of-care testing for disease diagnosis and health monitoring. (C) 2022 Published by Elsevier B.V. on behalf of Chinese Chemical Society and Institute of Materia Medica, Chinese Academy of Medical Sciences.