Hyperspectral Indices for Predicting Nitrogen Use Efficiency in Maize Hybrids

Enhancing the nitrogen (N) efficiency of maize hybrids is a common goal of researchers, but involves repeated field and laboratory measurements that are laborious and costly. Hyperspectral remote sensing has recently been investigated for measuring and predicting biomass, N content, and grain yield in maize. We hypothesized that vegetation indices (HSI) obtained mid-season through hyperspectral remote sensing could predict whole-plant biomass per unit of N taken up by plants (i.e., N conversion efficiency: NCE) and grain yield per unit of plant N (i.e., N internal efficiency: NIE). Our objectives were to identify the best mid-season HSI for predicting end-of-season NCE and NIE, rank hybrids by the selected HSI, and evaluate the effect of decreased spatial resolution on the HSI values and hybrid rankings. Analysis of 20 hyperspectral indices from imaging at V16/18 and R1/R2 by manned aircraft and UAVs over three site-years using mixed models showed that two indices, HBSI1 and HBS2, were predictive of NCE, and two indices, HBCI8 and HBCI9, were predictive of NIE for actual data collected from five to nine hybrids at maturity. Statistical differentiation of hybrids in their NCE or NIE performance was possible based on the models with the greatest accuracy obtained for NIE. Lastly, decreasing the spatial resolution changed the HSI values, but an effect on hybrid differentiation was not evident.