Role of increasing the immunity control the larvicidal effects of various essential against activity

The current study looks towards reporting the chemical compounds present in the panchagavya (PG), free radicals scavenging and mosquitocidal activity of PG in the laboratory condition. The existence of chemical compounds in the PG were studied by GC–MS analysis. Free radicals scavenging activity of PG was studied by using various invitro assays. Mosquitocidal efficacy of PG was studied by the experiment on larvicidal, pupicidal, adulticidal, fecundity, longevity, and ovicidal activity against An. stephensi , Ae. aegypti and Cx. quinquefasciatus. GC–MS analysis revealed fifteen chemical compounds present in the PG. Free radical scavenging was done by 2,2-diphenyl-1-picrylhydrazyl, 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid), hydroxyl, and superoxide assays, and the IC 50 was calculated as 37, 37.5, 35, and 38 μg/mL respectively. PG exhibited better larvae and pupae mortality against I-IV instar of Cx. quinquefasciatus (LC 50 : 148.765, 162.534, 187.619, 210.835 and 234.624 ppm, LC 90 : 286.636, 306.390, 350.276, 390.735 and 419.195 ppm). The highest adult mortality was found against An. stephensi (91.10 ± 1.74%) with the IC 50 and IC 90 values of 128.114 and 260.609 ppm. An. stephensi showed highly decreased fecundity and longevity even at a low concentration of PG. Inhibition of 100% egg hatchability of An. stephensi was obtained at 250 ppm followed by Ae. aegypti, and Cx. quinquefasciatus at 300 ppm respectively. On comparing with other mosquito vectors An. stephensi was effectively inhibited by PG at each stage of their life cycle. The results provide the first proof that PG could be a successful natural agent for controlling different mosquito vectors. Furthermore, our findings pave the way for more research into the efficacy of natural materials' mosquitocidal activities.