Fast large area multiphoton exoscope (FLAME) for improving the detection accuracy of early melanoma

Our group and others have demonstrated the strong potential of the multiphoton microscopy for a broad range of applications from advancing the understanding of skin biology to non-invasive diagnosis of skin diseases and monitoring therapy effects. We have recently reported on a fast large area multiphoton exoscope for rapidly mapping out macroscopic tissue areas with microscopic resolution and enhanced contrast for selective melanin detection. We will describe the technical abilities of this instrument and demonstrate its feasibility for early melanoma diagnosis based on a pilot study on ex-vivo and in-vivo imaging of pigmented lesions suspicious of melanoma in human skin.