In Vitro and in Vivo Assessment of Silver Nanoparticles and Sesame Oil combination as in Treatment of Sodium Nitrate-Induced Hepatotoxicity

The liver, which carries a variety of important functions, removes toxins and toxic chemicals from the body. "Hepatotoxic substances" can interact with basic cellular organelles, resulting in a wide range of liver disorders. The main goal of this study was to investigate if silver nanoparticles (Ag NPs) which prepared using Tri sodium citrate (TSC) and Ascorbic acid (AA) as reducing agents in oleic acid with different concentrations, sesame oil (SO) and their combination could protect albino rats from sodium nitrate (NaNO3) induced hepatotoxicity. UV-visible spectroscopy (UV-VIS) and transmission electron microscopy (TEM) were used to investigate the characteristics of the obtained Ag NPs. Changing the concentration of the reducing agent had no discernible impact. Ag NPs produced by TSC and AA were 28 nm and 60 nm in size on average, respectively. Following an antibacterial experiment against Escherichia coli and Pseudomonas aeruginosa. Sesame oil was combined with each concentration of Ag NPs. The antibacterial efficacy of a mixture of (SO) and Ag NPs 50 ppm versus Pseudomonas aeruginosa was higher than that of Escherichia coli. To evaluate the effect of sodium nitrate NaNO3 on the liver, 70 healthy adult male albino rats" were divided into seven groups, each with ten rats. G1 received a normal diet as a negative control, G2 received NaNO3 orally as a positive control to cause hepatotoxicity, G3 received (SO) only, G4 received Ag NPs 10 ppm only, Corn oil was replaced for six weeks with SO, Ag NPs10ppm, and a mixture of SO and Ag NPs10 ppm (1:1) + NaNO3 at a rate of 15 mg/kg. The results showed that combining sesame oil and Ag NPs (G7) enhanced liver (AST, ALT, GGT, ALK, albumin, total protein, and globuin) and kidney (urea and creatinine) functions, as well as oxidative stress biomarkers (MDA and GSH), The relative weight of the liver in G5 and G6 was restored to that of the normal control group. Furthermore, the histological structure of the liver was normal in comparison to the other treatment groups, with normal hepatocyte and hepatic vascular structure.