A multiple primers-mediated exponential rolling circle amplification strategy for highly sensitive detection of T4 polynucleotide kinase and T4 DNA ligase activity

Wanling Cui,Xiaoyang Fan,Congcong Shen,Qingshuai Chen,Xiumei Zhang,Enguang Lv,Chonghui Li, Qiang Li,Rui Wang,Shicai Xu,Jihua Wang

Microchemical Journal（2022）

引用 2|浏览12

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摘要

•A fluorescent method for detection of T4 PNK and T4 DNA ligase activity was proposed.•The method could realize label-free and highly sensitive detection of repair enzyme.•Multiple primers-mediated exponential rolling circle amplification was proposed.•The method could detect T4 PNK activity in HeLa cell lysate.

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关键词

T4 polynucleotide kinase,T4 DNA ligase,Exponential rolling circle amplification,Label-free,Fluorescent strategy

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