Rapid on-site detection of viable Escherichia coli O157: H7 in lettuce using immunomagnetic separation combined with PMAxx-LAMP and nucleic acid lateral flow strip

Yuanyi Wen, Youjiang Tan,Lichao Zhao,Xinrui Lv,Li Lin, Dezhi Liang, Li Wang

Microchemical Journal(2022)

引用 12|浏览5
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摘要
•A LAMP-based NALFS method for rapid on-site detection of viable E.coli O157: H7 was developed.•The method combined IMS and PMAxx to improve sensitivity and accuracy in lettuce samples.•The measurements can be completed within 2 h without any heavy and costly instruments.
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关键词
Viable Escherichia coli O157: H7,On-site detection,Immunomagnetic separation,Improved propidium monoazide,Nucleic acid lateral flow strip,Loop-mediated isothermal amplification
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