Assessment of diphenhydramine toxicity – Is its mode of action conserved between human and zebrafish?

•Diphenhydramine (DPH, 10 mg/L) reduced zebrafish embryos heartbeat rate (48 h);•DPH, 1 and 10 mg/L, induced larvae hypoactivity and erratic swimming, after 120 h;•DPH, 0.01 or 10 mg/L, reduced energy budgets and total glutathione content (96 h);•DPH affected cholinesterase/glutathione S-transferase activities and DNA integrity;•DPH target molecules are conserved in human and zebrafish.