Construction of Strand-seq libraries in open nanoliter arrays.
Cell reports methods(2022)
Abstract
Single-cell Strand-seq generates directional genomic information to study DNA repair, assemble genomes, and map structural variation onto chromosome-length haplotypes. We report a nanoliter-volume, one-pot (OP) Strand-seq library preparation protocol in which reagents are added cumulatively, DNA purification steps are avoided, and enzymes are inactivated with a thermolabile protease. OP-Strand-seq libraries capture 10%-25% of the genome from a single-cell with reduced costs and increased throughput.
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Key words
Strand-seq,haplotyping,inversions,nanoliter dispensing,one-pot library construction,single-cell sequencing,structural genomic variation,thermolabile protease
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